A standard methods, lactose-containing selective medium for selective detection of Coliform in water, dairy products and other food materials. The medium has been used for the determination of the coli-aerogenes content of water, milk and other dairy products, dairy equipment, and food products etc 1,2. Organisms which rapidly attack lactose produce purple colonies surrounded by purple haloes. Non-lactose or late-lactose fermenters produce pale colonies with greenish zones. Other related Gram-negative bacteria may grow but can be suppressed by incubation at >42 degrees C or by anaerobic incubation. Druce et al.3 found that Violet Red Bile Lactose Agar was as good an indicator of coli-aerogenes bacteria in milk as MacConkey Broth, and that the medium was suitable for determining the coli-aerogenes content of milk.
Appearance
Dehydrated Medium: Straw-pink colored, free-flowing powder.
Prepared medium: Dark purple colored gel
Storage conditions and Shelf Life
Store the dehydrated medium at 10-30 degrees C. Store the prepared medium at 2-8 degrees C and use as freshly as possible.