Viperin (Radical S-adenosyl Methionine Domain-containing Protein 2, Virus Inhibitory Protein, Endoplasmic Reticulum-associated, Interferon-inducible, Rsad2, Vig1)

Mouse Viperin is a 42kD protein belonging to the RSAD2 family. Viperin is the abbreviation of Virus inhibitory protein endoplasmic reticulum-associated interferon-inducible. It is an evolutionary conserved protein that is highly inducible by both type I and type II interferons. However, little or no induction by interferon gamma is observed in monocytic cell lines. Infection by many viruses, including human cytomegalovirus (hCMV), hepatitis C virus, yellow fever virus and Sendai virus, strongly induces viperin expression, suggesting a role in the host antiviral response. Viperin has also been shown to be important for the host anti-HIV responses. Furthermore, expression of viperin inhibits influenza replication by perturbing its release from the plasma membrane. Viperin expression alters plasma membrane fluidity by affecting formation of lipid rafts which are detergent-resistant membrane microdomains known to be the sites of influenza virus budding. HCMV infection induces the redistribution of viperin from the endoplasmic reticulum to the Golgi complex and ultimately to cytoplasmic vacuoles, suggesting that viperin may function at a distinct level in the viral lifecycle, at the point of glycosylated viral protein transport.\n\nApplications:\nSuitable for use in Western Blot, Flow Cytometry, Immunofluorescence and Immunoprecipitation. Not suitable for use in Functional Studies. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:50\nWestern Blot: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

V2115-80

Size

100ug

Applications

FC, IF, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1.Wang, X et al; The interferon-inducible protein viperin inhibits influenza virus release by perturbing lipid rafts. Cell Host Microbe 2007, 2: 96. 2. Lackman, R et al; Innate immune recognition triggers secretion of lysosomal enzymes by macrophages. Traffic 2007, 8: 1179.

Additional Info

Recognizes mouse Viperin. Species Crossreactivity: human.