VISA (Virus-Induced Signaling Adapter, Mitochondrial Antiviral Signaling Protein, MAVS, CARD Adapter Inducing Interferon-beta, Cardif, IPS-1)

VISA is an important protein required for innate immune defence against viruses. VISA acts downstream of DDX58 and IFIH1/MDA5, which detect intracellular dsRNA produced during viral replication, to coordinate pathways leading to the activation of NF-kappa-B, IRF3 and IRF7, and to the subsequent induction of antiviral cytokines such as IFN-beta and RANTES (CCL5). VISA is believed to activate the same pathways following detection of extracellular dsRNA by TLR3 and it has been hypothosized that VISA may protect cells from apoptosis. VISA is a ubiquitously expressed protein present in a range of cell types including T-cells, monocytes and epithelial cells, highest levels in tissues of the heart, skeletal muscle, liver, placenta and in peripheral blood leukocytes. Multiple isoforms of VISA have been identified.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-1ug/ml\nImmunohistochemistry (Paraffin): 2.5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies:\nSheep Anti Rabbit IgG HRP\n\nImmunohistochemistry Positive Control Tissue:\nMouse brain\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

V2124-20F

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Seth, R.B., et al. (2006) Antiviral innate immunity pathways. Cell Res. 16:141-7 2. Xu LG, et al. (2005) VISA is an adapter protein required for virus-triggered IFN-beta signaling. Mol. Cell 19:727-40. 3. Meylan E, et al. (2005) Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus. Nature 437(7062):1167-72.

Additional Info

Recognizes the Human Virus-induced-signalling-adaptor (VISA), also known as MAVS and KIAA1271, a protein of approximately 56.5kDa located on the mitochondrial membrane. Species crossreactivity: Mouse, rat.