Vitamin H, BioAssay(TM) ELISA Kit (Biotin, Coenzyme R, Bioepiderm)

Biotin (Vitamin H) serves as the prosthetic group of enzymes, which catalyze carboxylations in the organism. For this purpose, biotin is bound via its carboxy group to lysine residues of carboxylases, and the transfer of carbon dioxide takes place after its attachment to a nitrogen atom of biotin, forming the so-called active carbon dioxide. The awareness of the population for good health and its interest in healthy nutrition has increased significantly during the last years. After the content of vitamins in his nourishment has gained importance for the consumer, food has partially been vitaminized by the manufacturer. When there exists a lack of biotin, seborrhoea, dermatitis, anorexia, muscle pain, tiredness and nervous disorders can appear. Where biotin is synthesized by the human intestinal flora, deficiency symptoms are rare. Deficiency symptoms appear during excessive ingestion of raw egg white, which can be explained by its content of biotin-binding avidin.\n\nPrinciple of the Test:\nThis Biotin (Vitamin H) quantitative test is based on the principle of enzyme linked immunosorbent assay. Avidin, which shows a high affinity to biotin, is bound on the surface of a microtiter plate. Biotin containing samples or standards and a biotin-alkaline phosphatase conjugate are added into the wells of the microtiter plate. Enzyme-labeled and free biotin compete for the binding sites. After one hour incubation at room temperature, the wells are washed with diluted washing solution to remove unbound material. A substrate solution is added and incubated for 30 minutes, resulting in the development of a yellow color. The color development is inhibited by the addition of a stop solution. The yellow color is measured photometrically at 405nm. The concentration of biotin is indirectly proportional to the color intensity of the test sample.\n\nIntended Use:\nFor the quantitative determination of Biotin (Vitamin H) in food.\n\nSensitivity: \n0.5ng/ml\n\nRecovery: 98%\n\nIncubation Time: 90 minutes\n\nKit Components:\nV2143-80A: Microtiter plate, 1x96 wellls \nV2143-80B: Standard 0ng/ml, 1x500ul \nV2143-80C: Standard 1ng/ml, 1x500ul \nV2143-80D: Standard 2.5ng/ml, 1x500ul \nV2143-80E: Standard 5ng/ml, 1x500ul \nV2143-80F: Standard 10ng/ml, 1x500ul \nV2143-80G: Standard 25ng/ml, 1x500ul \nV2143-80H: Biotin-AP Conjugate (Biotin-Alkaline Phophatase), 1x15ml \nV2143-80J: Substrate Solution, 1x15ml \nV2143-80K: Stop Solution, 1x15ml \nV2143-80L: Standard/Sample Diluent, 2x50ml\nV2143-80M: Washing Solution 10X, 1x30ml\n\nStorage and Stability:\nStore all components at 4 degrees C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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