This elisa utilises specific binding between antibodies and vitellogenin (vtg) to quantify vtg in samples from medaka. the wells of microplates have been pre-coated with a specific capture antibody that binds to vtg in standard and sample added to the wells. a different vtg-specific detecting antibody, labelled with the enzyme horseradish peroxidase (hrp), is added to create a sandwich of vtg and antibodies. the enzyme activity is determined by adding a substrate that gives a coloured product, and the colour intensity is directly proportional to the amount of vtg present.
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