Vitronectin, Recombinant, Human

Vitronectin is a member of the pexin family. It is found in serum and tissues and promotes cell adhesion and spreading, inhbits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond. Extracellular matrix (ECM) proteins are produced intracellularly and are subsequently secreted into the surrounding cellular medium, actively regulating a diverse range of cell functions including cell adhesion, differentiation, proliferation, migration, invasion and survival. A primary utility of ECMs in in vitro culture is to promote cellular adhesion while maintaining cell viability and maximizing cell proliferation for downstream cell-based applications. Studies show that anchorage dependent cells growing on ECM undergo more efficient plating, have a higher proliferation rate, reach a higher density, and require lower serum and growth factor concentrations

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SPECIFICATIONS

Catalog Number

V2200-09B

Size

250ug

Applications

ELISA

Form

Supplied as a liquid in sodium phosphate, 150mM sodium chloride, pH 7.4, 0.1mM DTT, 0.1mM PMSF, 50% glycerol.

Purity

(same/more than)80% as shown by SDS-PAGE and Coomassie Blue staining.

References

1. Wojciechowski, K., et al., Prot. Expr. Purif. 36: 131-138, 2004. 2. Schvartz, I., et al., Int. J. Biochem. Cell Biol. 31: 539-544, 1999. 3. Wechsler-Reya, R.J., Trends Neurosci. 24: 680-682, 2001. 4. Mousa, S.A., Med. Res. Rev. 23: 190-199, 2003.