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Vitronectin, Recombinant, Human

Cat no: V2200-09C

Vitronectin, Recombinant, Human

Vitronectin is a large glycoprotein found in blood and the extracellular matrix (ECM). The gene for vitronectin encodes a 19 amino acid (aa) signal peptide and a 459 aa protein. The amino terminal 130 aa residues of vitronectin contains multiple binding sites for a variety of structures. Included is a site for binding to plasminogen activator inhibitor-1 (PAI-1) and urokinase receptor, an (RGD) sequence that binds avb3, avb5, aavb1, aIIbb3, avb6, and avb8 integrins, a stretch of acidic amino acids that includes two sulfated tyrosine residues that bind thrombin-anti-thrombin III complexes, and a collagen binding site. The major part of the vitronectin molecule (aa 132-459) contains six hemopexin-like repeats. The carboxyl-terminal end of vitronectin also has multiple sites and functions. It contains a stretch of basic amino acids that binds the acidic amino acids of the amino-terminal region, thereby stabilizing vitronectin

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SPECIFICATIONS

Catalog Number

V2200-09C

Size

50ug

Applications

ELISA

Form

Supplied as a lyophilized powder from 50mM Tris, 0.15M sodium chloride, pH 8.0. Reconstitute with 500ul sterile PBS, 0.1% HSA or BSA.

Purity

~90% as determined by SDS-PAGE and visualized by silver stain.

References

1. Schvartz, I., Seger, D., and S. Shaltiel, 1999, Int. J. Biochem. Cell Biol. 31:539 - 544. 2. http://www.copewithcytokines.de/cope.cgi

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