Vitronectin (Serum Spreading Factor, Complement S-Protein) is a plasma protein and a component of the extracellular matrix in many tissues, where it promotes cell adhesion and spreading. Vitronectin also inhibits complement C5b-9-mediated cytolysis and regulates the action of thrombin. Vitronectin contains the cell binding sequence arg gly asp (RGD) and serves as a cell-to-substrate adhesion molecule, (Stefansson, 1996).
Applications:
Suitable for use in Western Blot, Immunohistochemistry, and Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1:20,000
Immunohistochemistry (paraffin): 1:100-250
Immunocytochemistry: 1:50-100
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.