Vitronectin (Vitronectin V10 Subunit, Vitronectin V65 Subunit, VN, VNT, VTN, Complement S Protein, Epibolin, S Protein, S-Protein, Serum Spreading Factor, Somatomedin B, Somatomedin-B, V75)

Storage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

V2200-05

Size

100ug

Form

Purified human vitronectin in 0.15M sodium chloride, 0.005M HEPES, pH 7.4.

Purity

(same/more than)95% based on Coomassie brilliant blue stain of 10% SDS-PAGE. Human vitronectin was purified by the method of Hayman et al. using an \nanti-vitronectin monoclonal antibody affinity column. Purified protein sterile filtered (0.2um).

References

1. Hayman, E.G., M.D. Pierschbacher, Y. Ohgren, and E. Ruoslahti (1983) Serum spreading factor (vitronectin) is present at the cell surface and in tissues. Proc. Nat

Additional Info

Corning 24-well plates were coated with vitronectin in PBS (0.3 mL/well) at 37 degrees C overnight. After blocking with BSA, TIG-3 cells (5 x 10e4 cells/1 mL DMEM/well) were added and incubated at 37 degrees C for 90 minutes. Attached cells were counted electronically with a Coulter counter. Under these conditions, the use of a concentration of 3 mg vitronectin per milliliter resulted in the attachment of > 40% of the cells.