Vitronectin (Vitronectin V10 Subunit, Vitronectin V65 Subunit, VN, VNT, VTN, Complement S Protein, Epibolin, S Protein, S-Protein, Serum Spreading Factor, Somatomedin B, Somatomedin-B, V75)

Vitronectin is an abundant glycoprotein (~75kD), consisting of 459aa. About one third of the protein molecular mass is composed of carbohydrates. It is found in blood plasma and the extracellular matrix. Vitronectin is a multifunctional protein, since it promotes attachment and spreading of animal cells in vitro, inhibits cytolysis by the complement C5b-9 complex and modulates antithrombin III-thrombin action in blood coagulation. The protein consists of three domains: the N-terminal Somatomedin B domain (1-39), a central domain with hemopexin homology (131-342) and a C-terminal domain with hemopexin homology (347-459). The Somatomedin B domain binds to PAI-1 and is responsible for its stabilization. Furthermore, the Somatomedin B domain can also interact with the urokinase plasminogen activator receptor (uPAR). Vitronectin-uPAR interaction is required and sufficient to initiate downstream changes in cell morphology, migration and signal transduction. High plasma levels of both PAI-1 and uPAR have been shown to correlate with a negative prognosis for cancer patients. Additionally, vitronectin is a component of platelets and is as such involved in hemostasis. Aa45-47 (RGD) are capable of binding to membrane bound integrins, which serve to anchor cells to the extracellular matrix. Vitronectin in plasma is an inactive monomer form. In contrast, tissue vitronectin is an active multimeric form and is able to interact with various matrix ligands like proteoglycans and collagen. Mice with a genetic deletion of vitronectin show delayed wound healing, suggesting an important role of vitronectin in tissue remodeling after injury.\nApplications:\nSuitable for use in ELISA, Immunofluorescence, Western Blot Immunoprecipitation and Flow Cytometry . Other applications have not been tested.\n\nRecommended Dilution:\nImmunofluorescence: 1:50\nWestern Blot: 1:50\nFlow Cytometry: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

V2200-12B

Size

100ug

Applications

ELISA, FC, IF, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Martin-Padura, I et al; Expression of VE (vascular endothelial)-cadherin and other endothelial-specific markers in haomangiomas. J path 1995, 175: 51. 2. Zanetti, A et al; Clustering of vitronectin and RGD peptides on microspheres leads to engagement of integrins on the luminal aspect of endothelial cell membrane. Blood 1994, 84: 1116.

Additional Info

Binds to human vitronectin. It binds to soluble vitronectin as well as to membrane bound vitronectin.