Vitronectin (Vitronectin V10 Subunit, Vitronectin V65 Subunit, VN, VNT, VTN, Complement S Protein, Epibolin, S Protein, S-Protein, Serum Spreading Factor, Somatomedin B, Somatomedin-B, V75)

Vitronectin is a plasma glycoprotein that circulates in the blood. Vitronectin is circulating as a mixture of both 75kD and 65kD forms. Vitronectin is a major cell adhesive glycoprotein and is a common component of extracellular matrix and plasma. It competes effectively with other plasma proteins and is often involved in cell attachment, regulation of blood coagulation and immune responses. It has similar tissue distribution to fibronectin and also its integrin receptor recognises fibronectin.\n\nApplications: \nSuitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 1:80000 \nWestern Blot: 1:200\nImmunohistochemistry: 1:50 using frozen, PLP-fixed sections of bovine tissues.\nOptimal dilutions to be determined by the researcher.\n\nCulture Medium: \nRPMI 1640 with 10% fetal calf serum\n\nHybridoma: \nSp2/O myeloma cells with spleen cells from female BALB/c mice. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

V2200-15E

Size

100ul

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Bov, Sheep

Form

Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.

P Type

Mab

Purity

Purified by Protein A/G affinity chromatography.

Isotype

IgG1,k

References

1. Underwood PA, Bennett FA (1989) A comparison of the cell-adhesive proteins vitronectin and fibronectin. J Cell Sci 93:641-649. 2. Underwood PA, Steele JG, Dalton BA, Bennet FA (1990). Solid phase monoclonal antibodies. A novel method of directing the function of biologically active molecules be presenting a specific concentration. J Immunol Methods 127:91-102. 3. Underwood PA, Steele JG, Dalton BA (1993) Effects of polystyrene surface chemistry on biological activity of solid phase fibronectin and vitronectin, analysed with monoclonal antibodies. J Cell Sci 104:793-803. 4. Underwood PA, Bean PA, Mitchell SM, Whitelock JM (2001) Specific affinity depletion of cell adhesion molecules and growth factors from serum. J Immunol Methods 247:217-224.

Additional Info

Recognizes bovine vitronectin. Species Crossreactivity: sheep. Does not crossreact with human or equine. There is no evidence for crossreactivity with other connective tissue proteins (fibronectin, elastin, collagen, laminin).