Vitronectin (Vitronectin V10 Subunit, Vitronectin V65 Subunit, VN, VNT, VTN, Complement S Protein, Epibolin, S Protein, S-Protein, Serum Spreading Factor, Somatomedin B, Somatomedin-B, V75)

Vitronectin, a 75kDa polypeptide along with a 10kDa fragment, is a glycoprotein present on most cell surfaces, in extracellular fluids and plasma (1). Vitronectin promotes cellular adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway and binds to several serpin serine protease inhibitors (2). As a member of the integrin family, Vitronectin contains the cell binding sequence arg gly asp (RGD), first found in fibronectin, and serves as a cell-to-substrate adhesion molecule (3). Vitronectin acts as an inhibitor of the complement cascade by binding to the C5b9 complex and it has also been shown to have has a novel and direct role in self-senescent-self intercellular recognition leading to macrophage phagocytosis of cells undergoing apoptosis (4).\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:20,000\nImmunohistochemistry: 1:100- 1:250\nImmunocytochemistry: 1:50-1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

V2202E

Size

100ul

Applications

ICC, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 50 mM Tris- Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1.Rouslahti E, et al. J Invest Dermatol 79:65-68, 1982 2. Bauer JS, et al. J Cell Biol. 116(2):477-87, 19923. Stefansson S, et al. Nature 383(6599):390-1, 19964. Savill J, et al. Nature 343:170-3, 1990

Additional Info

Recognizes human Vitronectin. Species crossreactivity: mouse and rat.