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Von Willebrand Factor (vWF, Factor VIII Related Antigen) (BSA, Azide & Glycerol Free)

Cat no: V2700-08A

Von Willebrand Factor (vWF, Factor VIII Related Antigen) (BSA, Azide & Glycerol Free)

Von Willebrand Factor is synthesized by endothelial cells, causing adhesion of platelets to injured vessel walls and functions as a carrier and stabilizer for coagulation of Factor VIII. It is one of the most widely used endothelial cell markers for studying angiogenesis/neovascularization.\n\nApplications: \nSuitable for use in ELISA, RIA, Immunofluorescence, Western Blot, Immunocytochemistry, Immunohistochemistry and Immunoperoxidase staining. Other applications have not been tested.\n\nRecommended Dilutions:\nWestern Blot: Detected vWF on 10ug of human placenta lysates. \nImmunoperoxidase: Indirect staining\nImmunohistochemistry: Frozen and formalin fixed sections.\nELISA, RIA: Plasma\nImmunofluorescence: Tissues\nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies:\nI1904-02C: IgG, H&L (HRP) Pab Dk xMo \nI1904-03C: IgG, H&L (X-Adsorbed) (HRP) Pab Dk xMo\n\npI: 6.55\n\nSource: Ascites\n\nControl: Tonsil\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

V2700-08A

Size

250ul

Applications

ELISA, ICC, IF, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1

References

1. Francis, S.E., et al., Pathology 17: 579-585 (1985). 2. Exner, T., et al., Pathology 16: 356 (1984). 3. Francis, S., et al., Some studies with monoclonal antibodies to Human Factor VIII. Proceedings of the Haematology Society of Australia held in Perth, 1984.

Additional Info

Recognizes human von Willebrand Factor or Factor VIII related antigen at ~310kD in both plasma and tissues.

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