Von Willebrand Factor (vWF, Factor VIII Related Antigen) (HRP)

von Willebrand Factor (vWF, also previously referred to as Factor VIII related antigen) is a large adhesive protein produced in endothelial cells and megakaryocytes. There are two critical functions of vWF, the first being its involvement in the process of platelet adhesion and aggregation through interaction with platelet receptor glycoprotein Ib, the second being the binding and stabilization of Factor VIII (antihemophilic factor) for secretion and transport in plasma. The vWF precursor protein is synthesized with a 95,000D propeptide (also known as vWF antigen-II), believed to be involved in the intracellular multimerization of the vWF subunits. The mature vWF multimers are then packed into storage organelles within the cell (Weibel-Palade bodies) after which the propeptide is cleaved and released. vWF circulates as multimers of disulfide linked 220,000 dalton subunits and the molecular weight of these multimers ranges from 0.5-20 million daltons. The plasma concentration of vWF is typically 10ug/ml, but increased levels are often observed in pregnancy and other conditions of physiological stress. von Willebrand

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SPECIFICATIONS

Catalog Number

V2700-04B

Size

150ug

Applications

ELISA

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in a buffered stabilizer solution, 50% glycerol. Labeled with horseradish peroxidase (HRP).

P Type

Pab

Purity

Purified by adsorption before conjugation.

Isotype

IgG

References

US Biological application reference: Pipe, S.W. et al., (2011) J. Thrombosis and Haemostasis, DOI: 10.1111/j.1538-7836.2011.04505.x. 1. Montgomery, R.R. and Coller, B.S., Hemostasis and Thrombosis, 3rd Edition. eds. R.W. Colman, J. Hirsh, V.J. Marder, and E.W. Salzman, pp.134-168, J.B. Lippincott Co., Philadelphia, PA, USA (1994). 2. Sadler, J.E., JBC 266: 22,777-22,780 (1991). 3. Stableforth, et al., Brit Med J. 1: 994 (1976). 4. Budde, et al., Thrombosis & Haemostasis 63: 2 (1990).

Additional Info

Recognizes human vWF as demonstrated by ELISA and Immunoelectrophoresis.