VRL-1 (Transient receptor potential cation channel subfamily V member 2, TrpV2, osm-9-like TRP channel 2, OTRPC2, Vanilloid receptor-like protein 1, Stretch-activated channel 2B)

Capsaicin receptor, termed vanilloid receptor subtype 1 (VR1), has been cloned from sensory neurons. VR1 protein is a heat-gated cation channel. VR1 is also activated by noxious heat (elevation in temperature causing pain) and high proton concentrations (pH <6.0). Rat VR1 encodes a membrane protein of 838aa (~95kD) with 6 transmembrane domains. VR1 expression is limited to small to medium diameter primary sensory neurons. Recently, a structural homolog of VR1, termed VRL-1 (VR1 like proteins; rat 761aa, human 764aa) have been cloned and characterized. There is ~49% identity between VRL-1 and VR1. In contrast to VR1, VRL-1 does not respond to capsaicin, acid or moderate heat. Instead, it is activated by high temperatures with a threshold of ~52 degrees C. Within sensory ganglia, VRL-1 is most prominently expressed by a subset of medium to large-diameter neurons, and in many non-neuronal tissues (lung, spleen and intestine) that are unlikely to encounter temperatures of >50 degrees C. It is possible that VRL-1 is also activated by stimuli other than heat. \n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-10 ug/ml (ECL). Recombinant VR1 is ~90kD\nELISA: 0.5-1ug/ml. V2075-05A Control Peptide can be used to coat ELISA plates at 1ug/ml.\nImmunohistochemistry: 1-20ug/ml.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

V2075-05

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a lyophilized powder from PBS, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Carlton SM 2004, Pain, 10, 3, 616-627 -IHC. 2. Carlton SM 2001, Neuroscience Lett., 310, 53-56. -IHC. 3. Caterina MJ et al (1997) Nature 398, 436-441. 4. Kanzaki M et al (199) Nature Cell Biol. 1, 165-170.

Additional Info

Recognizes rat Vanilloid receptor like protein 1 (VRL-1). No significant sequence homology is found with VR1 or other proteins. Conserved 100% in mouse VRL-1 and growth factor regulated calcium channel. Species sequence homology: mouse 100% and human 50%.