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VSIG1 (V-set and Ig Domain-containing Protein 1, Glycoprotein 34, Cell surface antigen 33) (Biotin)

VSIG1 (V-set and Ig Domain-containing Protein 1, Glycoprotein 34, Cell surface antigen 33) (Biotin)

Cat no: V4965-24

Supplier: United States Biological
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VSIG1 (V-set and Ig domain-containing protein 1; also Glycoprotein 34 and Cell surface antigen 33) is a 55-70kD member of the JAM family of Ig-Superfamily proteins. It shows expression on gastric epithelium and testicular germ cells and likely participates in cell adhesion. Mouse VSIG1 precursor is 407 amino acids in length. It is a type I transmembrane glycoprotein that contains a 211aa extracellular domain (ECD) (aa23-234) and a 152aa cytoplasmic region. The ECD contains one V-type (aa23-134) and one C2-type Ig-like domain (aa145-229). Over aa23-234, mouse VSIG1 is 94% and 81% aa identical to rat and human VSIG1, respectively. There is one potential alternate start site at Met108. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.1-0.2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile TBS, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: V4965-24
Reactivities: Mouse, Rat
Hosts: Goat
Applications: Western Blot
Conjugates: Biotin
Size: 50ug
Form: Supplied as a lyophilized powder from 0.2um filtered solution in PBS, BSA. Labeled with Biotin. Reconstitute with 0.1% sterile BSA in TBS.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
Additional info: Recognizes the extracellular domain of mouse VSIG1. Species Crossreactivity: rat

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