VSIG1 (V-set and Ig Domain-containing Protein 1, Glycoprotein 34, Cell surface antigen 33) (Biotin)

VSIG1 (V-set and Ig domain-containing protein 1; also Glycoprotein 34 and Cell surface antigen 33) is a 55-70kD member of the JAM family of Ig-Superfamily proteins. It shows expression on gastric epithelium and testicular germ cells and likely participates in cell adhesion. Mouse VSIG1 \nprecursor is 407 amino acids in length. It is a type I transmembrane glycoprotein that contains a 211aa extracellular domain (ECD) (aa23-234) and a 152aa cytoplasmic region. The ECD contains one V-type (aa23-134) and one C2-type Ig-like domain (aa145-229). Over aa23-234, mouse VSIG1 is 94% and 81% aa identical to rat and human VSIG1, respectively. There is one potential alternate start site at Met108. \n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.1-0.2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile TBS, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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