VSIG2 (V-set and Ig domain-containing protein 2; also CTM and CT-like protein) is presumably a 50-60kD member of the CTX family of Ig-Superfamily proteins. It shows expression in stomach and prostate by Northern blot, and likely participates in cell adhesion. Mouse VSIG2 precursor is 328 amino acids (aa) in length. It is a type I transmembrane (glyco)protein that contains a 220 aa extracellular domain (ECD) (aa 25-244) and a 63 aa cytoplasmic region. The ECD contains one V-type (aa 25-138) and one C2-type Ig-like domain (aa 145-234). Over aa 25-244, mouse VSIG2 is 94%, 83% and 85% aa identical to rat, canine and human VSIG2, respectively. Two potential splice variants exist, one that shows a deletion of aa 305-328 and a second that shows a deletion of aa 144-236.
Applications:
Suitable for use in Direct ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
Direct ELISA: 0.5-1.0ug/ml detection limit for rmVSIG2 is ~ 1ng/well.
Western Blot: 0.1-0.2ug/ml detection limit for rmVSIG2 is ~ 1 ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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