VSIG8, CT (V-set and immunoglobulin domain containing 8)

VSIG8 protein expression was observed in the hair follicle at specific stages of the hair cycle, the oral mucosa, and the nail matrix. Expression in the hair follicle and shaft was found in the cuticle and cortex layers of the hair, where the labeling appeared intracellular and at the cell periphery. IHC analysis revealed that VSIG8 was located in the projections of the cortex between medullary cells, a poorly understood structure that is deficient in mice homozygous for the hair interior defect due to a mutation in Soat1 (Wu et al., 2010). To determine whether VSIG8 was expressed at all stages of the hair cycle or only in specific stages and anatomical sites, synchronized (wax-stripped) hair follicles (Sundberg and Silva, in press) were tested. Reactivity was restricted as we initially found in the hair follicle and hair shaft, and positive signals were limited to the late anagen and early catagen stages of the hair cycle.\n\nApplications:\nSuitable for use in Western Blot, ELISA.\n\nRecommended Dilution: \nELISA: 1:1,000\nWestern Blot: 1:100~800\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

031336

Size

100ul

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Mouse

Form

Supplied as a liquid, crude ascites with 0.09% sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgM

References

General References:\n1. Strausberg, R.L., et al. Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903(2002)\n2. Journal of Investigative Dermatology (2011) 131, 1936

Additional Info

Mouse