VspI

5'-A T^T A A T-3' \n3'-T A A T^T A-5'\n\nEnzyme Properties:\nStability during Prolonged Incubation: A minimum of 0.1 units of enzyme is required for complete digestion of 1ug of DNA in 16 hours at 37 degrees C. \n\nThermal Inactivation: Enzyme is inactivated by incubation at 65 degrees C for 20min. \n\nDigestion of Agarose-embedded DNA: A minimum of 5 units of enzyme is required for digestion of 1ug of agarose-embedded lambda DNA in 16 hours. \n\nCompatible Ends: Csp6I, MaeI, NdeI, Tru1I \n\nNumber of Recognition Sites in DNA:\nLambda: 17\nPhiX174: 2\nM13mp18/19: 7\npBR322: 1\npUC18/19: 3\npUC57: 3\npTZ19R/U: 3\npBluescriptIIKS(-/+): 3\npBluescriptIISK(-/+): 3\npACYC177: 2\npACYC184: 1\n\nSupplied With: Provided to simply buffer selection.\nR1625: Restriction Enzyme Buffer A, 10X.\nR1625-03: Restriction Enzyme Buffer D, 10X.\n\nDiluent Buffer: 10mM Tris-HCl (pH 7.4 at 25 degrees C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.\n\nStorage Buffer: 10mM Tris-HCl (pH 7.4 at 25 degrees C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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