Brorin (also VWC2, von Willebrand Factor C domain-containing protein 2) is a secreted, 48-49 kD member of the chordin family of proteins. It is expressed by neurons in multiple sites, and serves to sequester BMP family proteins such as BMP-2 and BMP-6. BMP antagonism is suggested to generate neurons from neural precursor cells at the expense of astrocytes. Human Brorin precursor is 325aa in length. It contains a 27aa signal sequence, one poly-Ala region (aa120-127), and two von Willebrand factor C domains (aa153-212 and 216-274) that likely serve as BMP interaction sites. Over aa28-325, human Brorin shares 90% aa identity with mouse Brorin.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA:0.5-1ug/ml
Western Blot:1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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