WASF1 (SCAR1, WAVE1, Wiskott-Aldrich syndrome protein family member 1, WAVE-1, WAVE)

The Wiskott-Aldrich syndrome protein (WASP) family plays essential roles in the regulation of actin polymerization. There are five WASP family members: Wasp, N-WASP, Wave1 (WASP family verprolin-homologous protein 1), WAVE2 and WAVE3. The Wasp family proteins share a conserved C-terminal region called the verprolin homology, cofilin homology and acidic domain (1, 2). The Wave/Scar family of prtoteins mediates signals to actin assembly by direct activation of the Arp2/3 complex. These proteins have been characterized as major regulators of lamellipodia formation downstream of Rac activation, and as members of large protein complexes. IT has been found that all three Scar/Wave isoforms behave similarily and are likely to participate in the same kinds of protein complexes (3).\n\nApplications: \nSuitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: 1-10ug/ml\nWestern Blot: 1ug/ml\nImmunohistochemistry: 0.1-1ug/ml\nImmunocytochemistry: 0.1-1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

136855

Size

100ug

Applications

ICC, IF, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.4, 50% glycerol.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Takenawa T. (2001) J Cell Sci. 114: 1801-1809. 2. Sarminto C., et al. (2008) JCB. 180(6): 1245-1260. 3. Stovold C.F., Millard T.H., and Machesky L.M. (2005) BMC Cell Biol. 6(1): 11.

Additional Info

Recognizes human WAVE1-Scar at ~75kD. Species Crossreactivity: Mouse and rat.

Alternative Names

SCAR1, WAVE1, Wiskott-Aldrich syndrome protein family member 1, WAVE-1, WAVE