WASP, N-, phosphorylated (Tyr-256) (WAS, THC, IMD2, WASP, Eczema-thrombocytopenia)

Members of the Wiskott-Aldrich sydrome protein (WASP) family regulate the formation of actin-based cell structures in many cell types. These proteins contain C-terminal actin-binding domains that can stimulate actin polymerization. WASP is expressed primarily in hematopoietic cells, while its homolog N-WASP is widely expressed. These proteins have 48% identity in human with higher homology in the functional regions of these proteins. Phosphorylation at serine and tyrosine residues regulates the activity of both proteins. WASP is tyrosine phosphorylated at tyrosine 291 after antigen receptor activation in B-cells and collagen stimulation of platelets. Phosphorylation of the analogous site in N-WASP (Tyr-256) stimulates its activity, reduces nuclear N-WASP, and is required for neurite extension.\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:10,000 \nELISA: 1:20,000\nImmunocytochemistry: 1:100\nOptimal dilutions to be determined by the researcher.\n\nPositive Controls:\nPervanadate treated A431, HeLa and endothelial cells.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

W0800-10

Size

100ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1999 Baba, Y. et al. Blood 93:2003.\n2003 Torres, E. & Rosen, M.K. Mol Cell 11:1215.\n2004 Wu, X. et al. J Biol Chem 279(10):9565.

Additional Info

Recognizes a 65kD protein corresponding to the molecular mass of human phosphorylated N-WASP. A similar band is observed in pervanadate treated HeLa and endothelial cells. Weak bands are also observed at a higher MW after pervanadate treatment, may be due to weak cross reactivity with phosphotyrosine.