Home  >  Products  >  WASP, N-, phosphorylated (Tyr256) (Neural Wiskott-Aldrich Syndrome Protein, DKFZp779G0847, MGC48327, Wiskott-Aldrich Syndrome Gene-like Protein, Wiskott Aldrich Syndrome Like, Wiskott-Aldrich Syndrome-like, WASL)
WASP, N-, phosphorylated (Tyr256) (Neural Wiskott-Aldrich Syndrome Protein, DKFZp779G0847, MGC48327, Wiskott-Aldrich Syndrome Gene-like Protein, Wiskott Aldrich Syndrome Like, Wiskott-Aldrich Syndrome-like, WASL)

WASP, N-, phosphorylated (Tyr256) (Neural Wiskott-Aldrich Syndrome Protein, DKFZp779G0847, MGC48327, Wiskott-Aldrich Syndrome Gene-like Protein, Wiskott Aldrich Syndrome Like, Wiskott-Aldrich Syndrome-like, WASL)

Cat no: W0800-02P

Supplier: United States Biological
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Members of the Wiskott-Aldrich sydrome protein (WASP) family regulate the formation of actin-based cell structures in many cell types. These proteins contain C-terminal actin-binding domains that can stimulate actin polymerization. WASP is expressed primarily in hematopoietic cells, while its homolog N-WASP is widely expressed. These proteins have 48% identity in human with higher homology in the functional regions of these proteins. Phosphorylation at serine and tyrosine residues regulates the activity of both proteins. WASP is tyrosine phosphorylated at tyrosine 291 after antigen receptor activation in B-cells and collagen stimulation of platelets. Phosphorylation of the analogous site in N-WASP (Tyr256) stimulates its activity, reduces nuclear N-WASP, and is required for neurite extension. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 1:20,000 Western Blot: 1:10,000 Optimal dilutions to be determined by the researcher. Positive Controls: Pervanadate treated A431, HeLa, Jurkat and endothelial cells for SDS PAGE immunoblots. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: W0800-02P
Reactivities: Human
Hosts: Rabbit
Applications: ELISA, Western Blot
Size: 50ul
Form: Supplied as a liquid in PBS, 1mg/ml BSA, 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Wu X et al. Focal adhesion kinase regulation of N-WASP subcellular localization and function. J Biol Chem 279:9565-76 (2004). 2. Torres E & Rosen MK Contingent phosphorylation/dephosphorylation provides a mechanism of molecular memory in WASP. Mol Cell 11:1215-27 (2003).
Additional info: Recognizes human WASP when phosphorylated at Tyr256.

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