Home  >  Products  >  WASP (Wiskott Aldrich Syndrome Protein, Wiskott-Aldrich Syndrome (Eczema Thrombocytopenia) Protein, WAS Protein, IMD2, Thrombocytopenia 1 (X-linked), THC1, THC)

WASP (Wiskott Aldrich Syndrome Protein, Wiskott-Aldrich Syndrome (Eczema Thrombocytopenia) Protein, WAS Protein, IMD2, Thrombocytopenia 1 (X-linked), THC1, THC)

Cat no: W0800-20E


Supplier: United States Biological
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Wiscott-Aldrich syndrome proteins (WASPs) mediate actin dynamics by activating the Arp2/3 actin nucleation complex in response to activated Rho family GTPases. In mammals, five WASP family members have been described. Hematopoietic WASP and ubiquitously expressed N-WASP are autoinhibited in unstimulated cells. Upon stimulation they are activated by cdc42, which relieves the autoinhibition in conjunction with phosphatidylinositol 4,5-bisphosphate. Three WAVE (Wasf, SCAR) family proteins are similar in sequence to WASP and N-WASP but lack the WASP/N-WASP autoinhibition domains and are indirectly activated by Rac (reviewed in 1). Both WASPand WAVE functions appear to be essential, as knockout of either N-WASP or Scar-2 in mice results in cardiac and neuronal defects and embryonic lethality (2,3). Loss of WASP results in immune system defects and fewer immune cells (4). WAVE-2 (WASF2) is widely distributed, while WAVE-1 and WAVE-3 are strongly expressed in brain (5). WAVE-3 may act as a tumor suppressor in neuroblastoma, a childhood disease of the sympathetic nervous system (6). Increased expression of WAVE-3 is seen in breast cancer, and studies in breast adenocarcinoma cells indicate that WAVE-3 regulates breast cancer progression, invasion and metastasis through the p38 mitogen-activated protein kinase (MAPK) pathway (7,8). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% w/c BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight. Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: W0800-20E
Reactivities: Human
Hosts: Rabbit
Applications: Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in PBS, 10mM sodium HEPES, 150mM NaCl, pH 7.5, 100ug/ml, 0.02% sodium azide, 1% BSA, 50% glycerol.
P type: Mab
Isotype: IgG
Purity: Supernatant
References: 1. Millard, T.H. et al. (2004) Biochem J. 380, 1-17, 2. Yan, C. et al. (2003) EMBO J. 22, 3602-3612, 3. Snapper, S.B. et al. (2001) Nat. Cell Biol. 3, 897-904, 4. Zhang, J. et al. (1999) J. Exp. Med. 190, 1329-4132, 5. Suetsugu, S. et al. (1999) Biochem. Biophys. Res. Commun. 260, 296-302, 6. Sossey-Alaoui, K. et al. (2002) Oncogene 21, 5967-5974, 7. Sossey-Alaoui, K. et al. (2005) Exp. Cell Res. 308,135-145, 8. Sossey-Alaoui, K. et al. (2007) Am J Pathol 170, 2112-21.
Additional info: Recognizes endogenous levels of total human WASP protein at 60kD.

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