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WC1 (Antigen WC1.1, CD163L1, CD163 Molecule-like 1)

Cat no: W0481-74

WC1 (Antigen WC1.1, CD163L1, CD163 Molecule-like 1)

WC1 expression appears to be heterogeneous and antibodies of this cluster show differing reaction patterns. CC101 immunoprecipitates a 215kD molecule from bovine cells, and also recognizes the swine homologue of WC1, which is a 180kD molecule. In pigs the 180kD molecule is expressed by a gamma/delta TCR positive T-cell population that is also CD2-, CD4-, CD8-.\n\nApplications:\nSuitable for use in Flow Cytometry, Immunohistochemistry (frozen), and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: Neat-1:10; 5ul labels 10e6 cells in 100ul\nImmunohistochemistry (frozen): Neat-1:5\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

W0481-74

Size

2ml

Applications

FC, IHC, IP

Hosts

Mouse

Reactivities

Bov, Prc

Form

Supplied as a liquid, 0.09% sodium azide.

P Type

Mab

Purity

Supernatant

Isotype

IgG2a

References

1. Carr, M.M. et al. (1994) Expression on porcine gamma/delta lymphocytes of a phylogenetically conserved surface antigen previously restricted in expression to ruminant gamma/delta T lymphocytes. Immunology 81: 36-40. 2. Howard, C.J. and Naessens, J. (1993) Summary of workshop findings for cattle Vet. Immunol. Immunopathol. 39: 25-48.

Additional Info

Recognizes bovine WC1 cell surface antigen, expressed by a population of gamma/delta TCR expression cells which lack CD2, CD4 and CD8 but express CD3. Species Crossreactivity: porcine.

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