WC1 expression appears to be heterogeneous and antibodies of this cluster show differing reaction patterns. This clone immunoprecipitates a 215 kD molecule from bovine cells, and also recognizes the swine homologue of WC1, which is a 180kD molecule. In pigs the 180kD molecule is expressed by a gamma/delta TCR positive T-cell population that is also CD2-, CD4-, CD8-.
Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry (frozen), and Immunoprecipitation. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 1:10-1:50; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.