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WC1 (Antigen WC1.1, CD163L1, CD163 Molecule-like 1)

Cat no: W0481-74A


Supplier: United States Biological
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WC1 expression appears to be heterogeneous and antibodies of this cluster show differing reaction patterns. This clone immunoprecipitates a 215 kD molecule from bovine cells, and also recognizes the swine homologue of WC1, which is a 180kD molecule. In pigs the 180kD molecule is expressed by a gamma/delta TCR positive T-cell population that is also CD2-, CD4-, CD8-. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry (frozen), and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:10-1:50; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: W0481-74A
Reactivities: Bovine, Porcine
Hosts: Mouse
Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation
Size: 250ug
Form: Supplied as a liquid, 0.09% sodium azide.
P type: Mab
Isotype: IgG2a
Purity: Purified by Protein G affinity chromatography.
References: 1. Carr, M.M. et al. (1994) Expression on porcine gamma/delta lymphocytes of a phylogenetically conserved surface antigen previously restricted in expression to ruminant gamma/delta T lymphocytes. Immunology 81: 36-40. 2. Howard, C.J. and Naessens, J. (1993) Summary of workshop findings for cattle Vet. Immunol. Immunopathol. 39: 25-48.
Additional info: Recognizes bovine WC1 cell surface antigen, expressed by a population of gamma/delta TCR expression cells which lack CD2, CD4 and CD8 but express CD3. Species Crossreactivity: porcine.

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