WC8, a cell surface antigen, is a 160kD protein which is expressed by activated bovine T cells. WC8 is not present on resting lymphocytes, but can be induced on T cells following activation by alloantigens or lectins (1).
Applications:
Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.
Hybridoma:
Spleen cells from immunized BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.