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WC8

WC8

Cat no: W0481

Supplier: United States Biological
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WC8, a cell surface antigen, is a 160kD protein which is expressed by activated bovine T cells. WC8 is not present on resting lymphocytes, but can be induced on T cells following activation by alloantigens or lectins (1). Applications: Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Hybridoma: Spleen cells from immunized BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: W0481
Reactivities: Bovine
Hosts: Mouse
Applications: Flow Cytometry, Immunoprecipitation
Size: 250ug
Form: Supplied as a liquid in PBS, pH7.4, 0.09 sodium azide.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography.
References: 1. Nthale, J.M. et al. (1993) Characterization of a late activation antigen defined by monoclonal antibodies of cluster BoWC8 (TC23). Vet. Immunol. Immunopathol. 39: 201-208. 2. Howard, C.J. et al. (1993) Summary of workshop findings for cattle (Tables 1 and 2). Vet. Immunol. Immunopathol. 39: 25-48.
Additional info: Recognizes the bovine WC8 cell surface antigen.

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