WC9 (FITC)

Cat no: W0482-01

Supplier: United States Biological

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Description
Additional Information

WC9 is expressed on bovine monocytes, platelets, granulocytes, peripheral B lymphocytes and a subset of T lymphocytes. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:10-1:50 10ul labels 1x10e6 cells in 100ul. Optimal dilutions to be determined by the researcher. Hybridoma: P3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Catalogue number:	W0482-01
Reactivities:	Bovine, Sheep
Hosts:	Mouse
Applications:	Flow Cytometry
Size:	100ug
Form:	Supplied as a liquid in PBS, pH7.4, 0.09% sodium azide, 1% BSA. Labeled with fluorescein isothiocyanate (FITC).
P type:	Mab
Isotype:	IgG1
Purity:	Purified by Protein G Chromatography.
References:	1. Naessens, J. et al. (1993) Biochemical characterization of three non-lineage antigens defined by workshop antibodies. Vet. Immunol. Immunopathol. 39: 217-223. 2. Menge, C. et al. (2004) Bovine Ileal Intraepithelial Lymphocytes Represent Target Cells for Shiga Toxin 1 from Escherichia coli. Infect. Immun. 72: 1896
Additional info:	Recognizes the bovine WC9 cell surface antigen. Species Crossreactivity: sheep.

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WC9 (FITC)

Cat no: W0482-01

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