WDR5 (WD repeat-containing protein 5; also BMP2-induced 3 kb gene protein/BIG3) is a
nuclear, 36-40kD monomeric member of the WD family of repeat proteins. It is known to be
expressed in osteoblasts, osteocytes and chrondrocytes, among other cells, and appears to
serve as a structural organizer for the histone methyltransferase MLL1 complex. This complex
methylates histone H3, and within this complex, WDR5 binds to mono- or dimethylated histone
H3 and serves as an anchor for complex subunits RbBP5, Ash2L and MLL1. Human WDR5 is
334 amino acids in length. It contains seven WD domains (aa43-333) that likely participate in protein-protein interactions, plus a phosphorylation site at Ser267. There are three isoform variants for WDR5. Human and mouse WDR5 are absolutely identical in aa sequence.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, sterile 40-50% glycerol, 0.02% sodium azide, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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