WNV belongs to the Flaviviridae family which includes closely viruses such as Dengue fever, Yellow fever and Tick borne encephalitis. West Nile Virus particles consist of a dense core made up of the core/capsid protein encapsulating the RNA genome surrounded by a membrane envelope containing envelope and matrix proteins. Research indicates that the viral core protein contributes to the WNV-associated inflammation via apoptosis induced though the caspase-9 pathway. West Nile Virus is believed to have originated in the West Nile region of Africa and has now spread to Europe, Asia and America where it has become a significant disease affecting human and domestic animal health.
Applications:
Suitable for use in ELISA, Immunofluorescence and Western Blot. Other applications not tested.
Recommended Dilutions:
ELISA: 1ug/ml
Immunofluorescence: 20ug/ml
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.