West Nile (WN), the most widespread among flaviviruses, was first isolated from the serum of a febrile woman in 1937 in the West Nile district ofuganda. West Nile virus was first detected in North America in 1999 and has subsequently spread throughout the United States and Canada and into Mexico and the Caribbean. In Africa, southern Europe, western Asia, and the United States, WNV has been isolated from mosquitoes of more than 40 species. In the United States, Canada, and Israel, WNV is responsible for significant avian mortality.
Applications:
Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry, Immunoprecipitation and Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Immunofluorescence: 1-2ug/ml
Western Blot: 0.5-2ug/ml
Immunohistochemistry (paraffin): 1ug/ml
Immunocytochemistry: 1-2ug/ml
Optimal dilutions to be determined by the researcher.
Positive Control:
West Nile virus infected cell lysate
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.