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West Nile Virus

Cat no: W1018-50N


Supplier: United States Biological
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West Nile Virus (WNV) is a member of the Flaviviridae, a plus-stranded virus family that includes St. Louis encephalitis virus, yellow fever virus, and Dengue virus. WNV was initially isolated in 1937 in the West Nile region of Uganda and has become prevalent in Africa, Asia, and Europe. It has rapidly spread across the United States with cases being observed in every continental state (reviewed in. Virus particles consist of a dense core made up of the core/capsid protein encapsulating the RNA genome surrounded by a membrane envelope embedded with envelope and matrix proteins which play a major role for WNV entry into target cells. The viral core protein is thought to contribute to the WNV-associated inflammation via apoptosis induced though the caspase-9 pathway as delivery of core gene delivery into the striatum of mouse brain and skeletal muscle resulted in cell death and inflammation. Applications: Suitable for use in Immunofluorescence and ELISA. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:10-1:50 ELISA: 1:20-1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: W1018-50N
Hosts: Mouse
Applications: ELISA, Immunofluorescence
Size: 100ug
Form: Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No Stabilizing proteins added.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein A affinity chromatography.
Additional info: Recognizes the envelope protein. Has positive reactivity with WNV and SLE flaviviruses. Has a negative reaction with JEV, YFV, DEN, TBE, EEE, WEE, POW, LAC, VEE.

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