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WIF1 (Wnt Inhibitory Factor 1, WIF-1, UNQ191/PRO217)

Cat no: W3005-75D


Supplier: United States Biological
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WIF1 (Wnt inhibitory factor 1) is a secreted protein that binds to Wnt proteins and inhibits their activity (1). It contains an N-terminal WIF domain and five EGF-like repeats (2). The WIF1 ortholog in Drosophila, Shifted, is required for Hedgehog stability and diffusion (3,4). It has been reported that WIF1 expression is downregulated in many types of cancers (5-8). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: W3005-75D
Reactivities: Human
Hosts: Rabbit
Applications: Western Blot
Size: 100ul
Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, <0.02% sodium azide, 50% glycerol.
P type: Mab
Isotype: IgG
Purity: Supernatant
References: 1. Hsieh, J.C. et al. (1999) Nature 398, 431-6. 2. Liepinsh, E. et al. (2006) J Mol Biol 357, 942-50. 3. Gorfinkiel, N. et al. (2005) Dev Cell 8, 241-53. 4. Glise, B. et al. (2005) Dev Cell 8, 255-66. 5. Wissmann, C. et al. (2003) J Pathol 201, 204-12. 6. Taniguchi, H. et al. (2005) Oncogene 24, 7946-52. 7. Mazieres, J. et al. (2004) Cancer Res 64, 4717-20. 8. Mazieres, J. et al. (2005) Cancer Lett 222, 1-10.
Additional info: Recognizes endogenous levels of total human WIF1.

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