WIPF1 (WAS/WASL interacting protein family, Member 1, MGC111041, PRPL-2, PRPL-2 protein, WAS/WASL interacting protein family member 1, WASP-interacting protein, WASPIP, WIP, Wiskott-Aldrich syndrome protein interacting protein, Wiskott-Aldrich syndrome pr

C-KLARNESRSGSNRRE, from the C-Terminus of human WIPF1.\nControl peptide corresponding to polyclonal antibody, Catalog#W0807-02, goat x human.\n\nThis gene encodes a protein that plays an important role in the organization of the actin cytoskeleton. The encoded protein binds to a region of Wiskott-Aldrich syndrome protein that is frequently mutated in Wiskott-Aldrich syndrome, an X-linked recessive disorder. Impairment of the interaction between these two proteins may contribute to the disease. Two transcript variants encoding the same protein have been identified for this gene.\n\nApplications:\nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilutions:\nOptimal dilutions to be determined by the researcher\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 monthsat -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nPeptide Blocking (see corresponding antibody W0807-02):\nAntibodies are typically supplied at 0.5mg/ml and peptides as a 100ul pellet. When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

W0807-02-P

Size

100ug

Applications

ELISA

Form

Supplied as a lyophilized powder in PBS. Reconstitution: Reconstitute with 200ul sterile ddH2O.

Purity

Highly Purified

References

Massarwa R, Carmon S, Shilo BZ, Schejter ED. WIP/WASp-based actin-polymerization machinery is essential for myoblast fusion in Drosophila. Dev Cell. 2007 Apr;12(4):557-69.