WNK1 (Kinase deficient protein, Protein kinase lysine deficient 1, Protein kinase with no lysine 1, Serine/threonine protein kinase WNK1)

Supplier:

United States Biological

Cat no: W2996-03

WNK1 (Kinase deficient protein, Protein kinase lysine deficient 1, Protein kinase with no lysine 1, Serine/threonine protein kinase WNK1)

The WNK [with no lysine (K)] family of serine/threonine kinases is characterized by having a cysteine in place of lysine in subdomain II of its kinase activation domain (1,2). The lysine necessary for phosphoryl transfer is located in an atypical position in the catalytic domain. Four members have been identified in humans (WNK 1-4) and have been implicated in regulating ion permeability (3). Mutations in the WNK1 and WNK4 genes in humans cause pseudohypoaldosteronism type II (PHAII), an autosomal dominant disorder leading to hypertension, hyperkalemia, and renal tubular acidosis (4). WNK4 is specifically expressed in the kidney, whereas WNK1 has a wider distribution but is predominantly expressed in polarized epithelia (1-3). Heterozygous mutations in WNK1 in mice result in a significant decrease in blood pressure, while homozygous mutations are embryonic lethal (5). WNK1 is phosphorylated by Akt at Thr60 (6). In addition, WNK1 may be autophosphorylated at Ser382 in the activation loop, and this is thought to be required for its kinase activity (7).\n\nApplications: \nSuitable for use in Western Blotting, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blotting: 1:1000\nImmunocytochemistry: (IF) 1:500\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot. Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

W2996-03

Size

100ul

Applications

ICC, WB

Hosts

Rabbit

Reactivities

Hum, NHP

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 100ug/ml BSA, 50% glycerol.

P Type

Pab

Purity

Protein A and peptide affinity chromatography.

References

(1) Verissimo, F. and Jordan, P. (2001) Oncogene 20, 5562

Additional Info

Detects endogenous levels of total WNK1 protein. Species Crossreactivity: Human Monkey

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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