Wnt-1 (Wingless-type MMTV Integration Site Family Member 1, WNT1, INT1, Proto-oncogene Int-1 Homolog, Proto-oncogene Wnt-1)

Supplier:

United States Biological

Cat no: W3035-25D

Wnt-1 (Wingless-type MMTV Integration Site Family Member 1, WNT1, INT1, Proto-oncogene Int-1 Homolog, Proto-oncogene Wnt-1)

Wnt-1 is a secreted protein that signals through the Frizzled family of cell surface receptors and is required for normal embryonic development. Wnt-1 activation induces a complex signaling cascade that ultimately leads to the increased expression of over fifty genes. An important component of Wnt-1 signaling is the stabilization, and resulting accumulation, of the intracellular signaling protein, beta-catenine. Wnt signaling induces and maintains the transformed phenotype and, in certain embryonic cell lines, supports self renewal in the absence of significant differentiation. Elevated levels of Wnt proteins are associated with tumorigenesis and are present in numerous human breast cancers. Mature human Wnt-1 is a glycosylated protein containing 343 amino acid residues.\n\nApplications:\nSuitable for use in Western Blot. Other applications have not been tested. \n\nRecommended Dilution: \nWestern Blot: 0.5-4ug/ml\nOptimal dilutions to be determined by the researcher. \n\nPositive Control:\nRecombinant human Wnt-1\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

W3035-25D

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.5% BSA, 0.01% thimerosal, 30% glycerol.

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

Additional Info

Recognizes human Wnt-1.

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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