Wnt Inhibiting Factor 1 (WIF-1)

Cat no: W3005-75

Supplier: United States Biological

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WIF-1 is a secreted protein that binds Wnt proteins and antagonizes Wnt-induced signaling. WIF-1 expression is down regulated in several human cancers. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml Immunohistochemistry (Paraffin): 8-25ug/ml; Used to detect WIF-1 in immersion fixed paraffin-embedded human breast sections. Optimal dilutions to be determined by the researcher. Hybridoma: Mouse myeloma with B cells from a mouse immunized with purified, NS0-derived, recombinant human WIF-1. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Catalogue number:	W3005-75
Reactivities:	Human
Hosts:	Mouse
Applications:	Immunohistochemistry, Western Blot
Size:	50ug
Form:	Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 100ul sterile PBS.
P type:	Mab
Isotype:	IgG1
Purity:	Purified by Protein G affinity chromatography.
Additional info:	Recognizes human Wnt Inhibiting Factor 1. Shows less than 5% crossreactivity with recombinant mouse WIF-1.

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Wnt Inhibiting Factor 1 (WIF-1)

Cat no: W3005-75

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