Wnt1, a 40kD cyteine-rich secretory glycoprotein, is a highly conserved archetypal member of WNT protein family. Wnt1, originally identified as proto-oncogene, plays a significant role in oncogenesis and in various developmental processes including regulation of cell fate, cell survival and patterning during embryogenesis. Wnt1 binds to its FZD receptor on the cell surface resulting in activation of beta-Catenin and translocation into the nucleus. Activated beta-catenin makes a complex with TCF family of transcription factors in the nucleus resulting in transactivation of several genes including c-myc and Cyclin D1 who play pivotal role in cell survival and differentiation. Mutations in the members of Wnt1 signaling have been suggested in several cancer types.
Applications:
Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunofluorescence: 1:200-1:1000
Flow Cytometry: 1:200-1:400
ELISA: 1:10,000
Western Blot: 1:500-1:2000
Immunohistochemistry (Formalin fixed paraffin embedded): 1:200
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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