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Wnt2B (Wnt13, Protein Wnt-2b, Protein Wnt-13, WNT2B Wingless-type MMTV Integration Site Family Member 2B)

Wnt2B (Wnt13, Protein Wnt-2b, Protein Wnt-13, WNT2B Wingless-type MMTV Integration Site Family Member 2B)

Cat no: W3035-40B

Supplier: United States Biological
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Wnt2b is a signalling factor which acts as a ligand for members of the frizzled family of seven transmembrane receptors. It is thought to play a key role during mammalian early embryogenesis, and may also function as a stem cell factor for neural and retinal progenitor cells. The Wnt2b gene encodes two alternative transcript variants; Wnt2B splicing variant 1 encodes secreted-type glycoprotein (Wnt2B isoform 1), while Wnt2B splicing variant 2 encodes a transmembrane-type glycoprotein (Wnt2B isoform 2). Wnt2b has been implicated in gastric cancer, esophageal cancer and skin basal cell carcinoma. Applications: Suitable for use in Immunohistochemistry (paraffin). Other applications not tested. Recommended Dilution: Immunohistochemistry (paraffin): 5-10ug/ml Requires antigen retrieval using heat treatment for 20 minutes prior to staining of paraffin sections. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: W3035-40B
Reactivities: Human
Hosts: Rabbit
Applications: Immunohistochemistry
Size: 50ug
Form: Supplied as a liquid in PBS, 0.09% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Katoh M. (2005) WNT2B: comparative integromics and clinical applications. Int. J. Mol. Med. 16: 1103-1108.
Additional info: Recognizes epitope within the internal region (IN) of human Wnt2b. Species sequence homology: mouse, rat.

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