WNT3a, Recombinant, Mouse (Protein Wnt-3a, Wnt-3a)

Wnt-3a belongs to the Wnt family of signaling proteins that play a key role in maintaining the integrity of embryonic and adult tissues. Expression of Wnt-3a occurs primarily along the dorsal midline across overlapping regions of the Central Nervous System (CNS). Wnt-3a signaling is essential for various morphogenetic events including embryonic patterning, cell determination, cell proliferation, CNS development, and cytoskeletal formation. Like other members of this family, Wnt-3a contains a highly conserved lipid modified cysteine rich domain that is essential for cell signaling. During a biochemical process called the canonical Wnt pathway; Wnt family members bind to and activate seven-pass transmembrane receptors of the Frizzled family ultimately leading to the disruption of b-Catenein degradation. Intracellular accumulation of b-Catenin increases translocation of the protein into the nucleus where it binds to TCF/LEF transcription factors to promote gene expression. Lack of Wnt signaling disrupts transcriptional activation of tumor suppressor genes and has shown to result in neoplastic transformation, oncogenesis, and human degenerative diseases. Recombinant murine Wnt-3a is a monomeric glycoprotein containing 328aa. Due to glycosylation, the murine Wnt-3a migrates at an apparent molecular weight of approximately 38-41kD by SDS-PAGE analysis under non-reducing conditions. Source: Recombinant protein corresponding to mouse WNT-3a, expressed in cell culture. Biological Activity: Determined by its ability to induced alkaline phosphatase production by CCL-226 cells. The expected ED50 for this effect is 0.4-0.6ng/ml. Endotoxin: <0.1ng/ug (1EU/ug) AA Sequence: SLAVGPQYSS LSTQPILCAS IPGLVPKQLR FCRNYVEIMP SVAEGVKAGI QECQHQFRGR RWNCTTVSNS LAIFGPVLDK ATRESAFVHA IASAGVAFAV TRSCAEGSAA ICGCSSRLQG SPGEGWKWGG CSEDIEFGGM VSREFADARE NRPDARSAMN RHNNEAGRQA IASHMHLKCK CHGLSGSCEV KTCWWSQPDF RTIGDFLKDK YDSASEMVVE KHRESRGWVE TLRPRYTYFK VPTERDLVYY EASPNFCEPN PETGSFGTRD RTCNVSSHGI DGCDLLCCGR GHNARTERRR EKCHCVFHWC CYVSCQECTR VYDVHTCK Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.