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Wnt3a (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member)

Wnt3a (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member)

Cat no: W3011-02C

Supplier: United States Biological
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The Wnt family includes several secreted glycoproteins that play important roles in animal development (1). There are 19 Wnt genes in the human genome that encode functionally distinct Wnt proteins (2). Wnt members bind to the Frizzled family of seven-pass transmembrane proteins and activate several signaling pathways (3). The canonical Wnt/b-catenin pathway also requires a coreceptor from the low-density lipoprotein receptor family (4). Aberrant activation of Wnt signaling pathways is involved in several types of cancers (5). Wnt3a protein is palmitoylated and can function as a growth factor for hematopoietic stem cells (6). Although functionally distinct, Wnt3a shows high homology to Wnt3 (7). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot (transfected): 1:1000. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: W3011-02C
Reactivities: Human, Mouse
Hosts: Rabbit
Applications: Western Blot
Size: 100ul
Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
References: 1. Cadigan, K.M. and Nusse, R. (1997) Genes Dev. 11, 3286-3305. 2. Moon, R.T. et al. (1997) Trends Genet. 13, 157-162. 3. Kohn, A.D. and Moon, R.T. (2004) Cell Calcium 38, 439-446. 4. Logan, C.Y. and Nusse, R. (2004) Annu. Rev. Cell Dev. Biol. 20, 781-810. 5. Giles, R.H. et al. (2003) Biochim. Biophys. Acta. 1653, 1-24. 6. Willert, K. et al. (2003) Nature 423, 448-52. 7. Katoh, M. (2001) Int. J. Oncol. 19, 977-82.
Additional info: Recognizes transfected human Wnt3a protein in L cells and the conditioned medium from L/Wnt3a cells. Based on homology, it is expected to also recognize Wnt3. Species Crossreactivity: mouse.

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