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Wnt4 (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member)

Wnt4 (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member)

Cat no: W3021A

Supplier: United States Biological
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Wnt-4 is a secreted glycoprotein that binds to receptors of the Frizzled family. Wnt-4 is important for multiple embryonic development processes including kidney morphogenesis. Applications: Suitable for use in Direct ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.5-1.0ug/ml. The detection limit is ~1ng/well. Western Blot: 5ug/ml. The detection limit is ~1ng/lane under non-reducing and reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: W3021A
Reactivities: Human, Mouse
Hosts: Rat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
P type: Mab
Isotype: IgG2a
Purity: Purified by Protein G affinity chromatography.
Additional info: Recognizes mouse Wnt-4. Species crossreactivity: human. Does not cross-react with recombinant mouse Wnt-1, 2b, 5a, 5b, 8a, 8b, 9b, 10a, 10b, 11, 16, or with recombinant human Wnt-2, 3a, 7a, or 7b.

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