Wnt6 (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family, Member 6 (Wnt6)

Wnts have diverse roles in governing cell fate, proliferation, migration and polarity during development, particularly in embryogenesis, and also play a role in carcinogenesis in adults1-3. Wnts are transduced through at least three distinct intracellular signaling pathways, including the canonical Wnt/b-catenin, Wnt/Ca2+ and Wnt polarity (also called

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SPECIFICATIONS

Catalog Number

W3036

Size

100ug

Applications

IF, IHC, WB

Hosts

Rabbit

Reactivities

Ch/Bird

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Pab

Purity

Epitope-affinity purified from rabbit antiserum.

Isotype

IgG

References

1. Cadigan KM and Nusse R. Genes Dev 11:3286-3305, 1997. 2. Miller JR, et al. Oncogene 18:7860-7872, 1999. 3. Polakis P. Genes Dev 14:1837-1851, 2000. 4. Adler, PN and Lee H. Curr Opin Cell Biol 13:635-640, 2001. 5. Kuhl M, et al. Trends Genet 16:279-283, 2000. 6. Rodriguez-Niedenfuhr M, et al. Anat Embryol (Berl) 206:447-451, 2003. 7. Garcia-Castro MI, et al. Science 297:848-851, 2002. 8. Schubert FR, et al. Mech Dev 114:143-148, 2002. 9. Dietrich S, et al. Development 125:2235-2249, 1998.

Additional Info

Reacts with the chicken Wnt6 protein. On Western blots, it identifies a band at ~35kD and one at ~45kD. The two bands may represent different isoforms or post-translational modification states. Immunohistochemical and immunofluoresent staining using this antibody is observed in non-neural ectoderm of chick embryos from stages 8 to 14, consistent with the reported expression of Wnt6 mRNA as determined by in situ hybridization. Additional staining is found in ectodermal tissues caudal to the otic level in heart- and gut-forming regions. Reactivity has been confirmed with stage 8-20 chicken embryo homogenates.