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Wnt6 (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family, Member 6 (Wnt6)

Cat no: W3036-01

Wnt6 (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family, Member 6 (Wnt6)

Human Wnt-6 is a 46 kD, secreted, glycoprotein of the Wnt family. Human Wnt-6 is synthesized as a 365 amino acid (aa) precursor that contains a 341 aa mature region. The mature molecule contains 24 cysteines and one potential N-linked glycosylation site. Wnt-6 is produced by mammary fibroblasts and ductal epithelium, intestinal crypt cells and limb bud ectoderm. Over the regions used as immunogen, the aa sequences of human, mouse and canine Wnt-6 are identical.\n\nApplications: \nSuitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.1-0.2ug/ml\nImmunohistochemistry: 5-15ug/ml\nELISA: 0.5-1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

W3036-01

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Sheep

Reactivities

Hum

Form

Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile, ddH2O or sterile 40-50% glycerol.

P Type

Pab

Purity

Purified by human Wnt-6 affinity chromatography.

Isotype

IgG

Additional Info

Recognizes human Wnt-6.

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