Wnt8b (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member)

Mouse Wnt-8b is a 40-50kD secreted glycoprotein member of the Wnt family of proteins. Mature mouse Wnt-8b is 329aa in length and contains 24 cysteines plus three potential N-linked glycosylation sites. Little is known about Wnt8b. It appears to play a role in forebrain development.\n\nApplications:\nSuitable for use in Immunohistochemistry, Western Blot, and ELISA. Other applications not tested.\n\nRecommended Dilutions: \nImmunohistochemistry: 10ug/ml detects Wnt-8b on mouse fetal brain tissue sections. Sections were fixed with PBS containing 4% paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted W3037-10 overnight at 4 degrees C followed by Rhodamine Red-coupled anti-rat IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA.\nWestern Blot: 1-2ug/ml with the appropriate secondary reagents detects human or mouse Wnt-8b. Using a colorimetric detection system, the detection limit is ~25ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold.\nDirect ELISA: 0.5-1.0ug/ml. The detection limit is ~5ng/well. \nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute by adding sterile 40-50% glycerol, PBS. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

W3037-10

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Rat

Reactivities

Hum, Mouse

Form

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile 40-50% glycerol, PBS.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

Additional Info

Recognizes mouse Wnt-8b in direct ELISAs and Western Blots. Western Blots with human cell extracts demonstrate that this antibody also detects human Wnt-8b Does not crossreact with rmWnt-1, -2b, -3a, -4, -5a, -5b, -8a, -9b, -10b, rhWnt-2, -7a, -7b, or -9a. Species sequence homology: rat 100%, canine 98% and human 97%.