Wingless-type MMTV integration site family, member 9A, also known as WNT9A or WNT14, is a human gene. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes,including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is expressed in gastric cancer cell lines. The protein encoded by this gene shows 75% aa identity to chicken Wnt14, which has been shown to play a central role in initiating synovial joint formation in the chick limb. This gene contains 4 exons and spans about 27kb of genomic DNA, in the chromosome 1q42 region.
Applications:
Suitable for use in Western Blot and Immunohistochemistry (paraffin). Other applications not tested.
Recommended Dilution:
Western Blot:1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile dH20. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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