Wnt9b, Recombinant, Mouse (Carrier Free) (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member)

Mouse Wnt-9b, previously called Wnt-14b or Wnt-15, is one of at least 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved, cysteine-rich secreted glycoproteins that are important for normal developmental processes (1-3). Wnts \nmainly transduce signals by binding to receptors of the Frizzled family, in conjunction with a coreceptor of the low-density lipoprotein receptor-related protein family (LRP-5 or -6) (1, 2). The 359 aa mouse Wnt-9b precursor contains a 336aa mature region with 24 conserved cysteines (3). Mature mouse Wnt-9b shows 93%, 98%, 92%, 92% and 91% aa identity with human, rat, canine, equine and bovine Wnt-9b, respectively. It is evolutionarily related to Wnt-9a and Wnt-3, which share 63% and 32% aa identity with Wnt-9B, respectively (4). Wnt-9b mRNA is expressed in late embryos and in adult kidney, with lesser amounts in brain and liver (3, 4). It appears to \ndirect a mesenchymal-to-epithelial transition in the kidney and other tissues (5). During kidney development, it is expressed in the ureteric bud and induces mesonephric and metanephric tubulogenesis, nephron development, and caudal extension of the Mullerian duct, acting upstream of Wnt-4 (5-7). Induction is dependent on beta-catenin activity, implicating the canonical signaling pathway (7). Mice devoid of Wnt-9b die shortly after birth due to kidney agenesis, while low expression results in small kidneys with fewer nephrons (1, 5, 6). Mutations \nof Wnt-9b also cause incompletely penetrant cleft lip and palate in mice, indicating its involvement with facial midline morphogenesis (8, 9). It has weak transforming activity compared to other transforming Wnts (4). \n\nSource: \nA DNA sequence encoding mouse Wnt-9b (Met 1-Arg 359; O35468) was expressed in Chinese Hamster Ovary cells.\n \nMolecular Mass: \nBased on N-terminal amino acid sequencing, the recombinant mouse Wnt-9b starts at Ala 24 and has a calculated molecular mass of approximately 36.8kD. The recombinant mouse Wnt-9b \nmigrates as an approximately 39-41kD protein in SDS-PAGE under reducing conditions.\n \nActivity: \nMeasured by its ability to induce alkaline phosphatase synthesis by C3H10T1/2 cells (ATCC# CCL-226). The typical ED 50 range is 100-400ng/ml. \n\nEndotoxin: (same/less than)1.0 EU/1ug (LAL)\n\nStorage and Stability:\n12 months from date of receipt, 20 to 70 degrees C as supplied.\n1 month, 2 to 8 degrees C under sterile conditions after reconstitution.\n3 months, 20 to 70 degrees C under sterile conditions after reconstitution.\nUse a manual defrost freezer and avoid repeated freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

W3037-60C

Size

25ug

Form

Supplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS, 0.1mm EDTA, 0.5% CHAPS, pH 6.8. Reconstitute with 40-50% glycerol, PBS.

Purity

(same/more than) 95% by SDS

References

1. www.stanford.edu/~rnusse/wntwindow.html 2. Logan, C. Y. & R. Nusse, 2004, Annu. Rev. Cell Dev. Biol. 20:781. 3. Kirikoshi, H. & M. Katoh, 2002, Int. J. Mol. Med. 9:135. 4. Qian, J. et al., 2003, Genomics 81:34. 5. Carroll, T. J. et al., 2005, Dev. Cell 9:283. 6. Merkel, C. E. et al., 2007, Pediatr. Nephrol. 22:1825. 7. Park, J-S. et al., 2007, Development 134:2533. 8. Lan, Y. et al., 2006, Dev. Dyn. 235:1448. \n9. Juriloff, D. M. & M. J. Harris, 2008, Birth Defects Res. A Clin. Mol. Teratol. 82:63.