WSTF (Williams Syndrome Transcription Factor, BAZB1, Bromodomain Adjacent to Zinc Finger Domain 1B, WBSCR9, Williams-Beuren Syndrome Chromosome Region 9, WBSC10, Williams-Beuren Syndrome Chromosone)

The human WSTF gene is located within the common Williams Syndrome (WS) deletion area at chromosome 7q11.23. Several WSTF gene products have been detected with little difference in length of polypeptides (1-3). Functional motifs identified by sequence-homology searches include a PHD-type zinc finger motif followed by a bromodomain. Both motifs are found in many transcriptional factors, suggesting that WSTF may function as a transcription factor. A Drosophila gene (acf1) was cloned, which encodes two forms of Acf1 proteins with molecular weight 170kD and 185kD, respectively (4). It was demonstrated that Acf1 is structurally related with the human WSTF gene. Acf1 forms a complex with another protein, ISWI, and functions in the ATP-dependent catalysis of chromatin assembly (4). \n\nApplications: \nSuitable for use in ELISA, Western Blot, and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nImmunoprecipitation: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

W1107

Size

100ul

Applications

ELISA, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, NHP

Form

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Peoples, R. J. et al. (1998) Cytogenet. Cell Genet. 82, 238

Additional Info

Detects endogenous levels of total human WSTF protein. Does not crossreact with other related transcriptional factors. Species Crossreactivity: Human, monkey, mouse and rat.