WT1, ID (E361) (WT1, Wilms tumor protein, WT33) (Biotin)

This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation site upstream of and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq].\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:100-500\nImmunohistochemistry: 1:50-100\nImmunofluorescence: 1:10-50\nFlow Cytometry: 1:10-50\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

043900-Biotin

Size

200ul

Applications

ELISA, FC, IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Conjugates

Biotin

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Biotin.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

Sitaram, R.T., et al. Br. J. Cancer 103(8):1255-1262(2010)\nDohi, S., et al. Anticancer Res. 30(8):3187-3192(2010)\nRocquain, J., et al. BMC Cancer 10, 401 (2010) :\nWagner, K.D., et al. J. Cell. Sci. 116 (PT 9), 1653-1658 (2003) :\nMitsuya, K., et al. Hum. Mol. Genet. 6(13):2243-2246(1997)

Additional Info

Human

Alternative Names

WT1, Wilms tumor protein, WT33