WWTR1, CT (WWTR1, TAZ, WW domain-containing transcription regulator protein 1, Transcriptional coactivator with PDZ-binding motif) (Biotin)

Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. Regulates the nuclear accumulation of SMADS and has a key role in coupling them to the transcriptional machinery such as the mediator complex. Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition.\n\nApplications:\nSuitable for use in Western Blot, ELISA\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:100-500\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

043909-Biotin

Size

200ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Conjugates

Biotin

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Biotin.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

Remue, E., et al. FEBS Lett. 584(19):4175-4180(2010)\nStrakova, Z., et al. Biol. Reprod. 82(6):1112-1118(2010)\nCho, H.H., et al. J. Cell. Physiol. 223(1):168-177(2010)\nMcGeachie, M., et al. Circulation 120(24):2448-2454(2009)\nFerrara, A.M., et al. J. Endocrinol. Invest. 32(3):238-241(2009)

Additional Info

Human

Alternative Names

WWTR1, TAZ, WW domain-containing transcription regulator protein 1, Transcriptional coactivator with PDZ-binding motif